MGMT antibody

Catalog No. ABIN7249814

Product Details anti-MGMT Antibody

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Recombinant protein corresponding to Mouse MGMT

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 260 μg/mL

Buffer: PBS with 0.02 % sodium azide, 1 % BSA and 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT (MGMT Products)

Synonyms: AGT antibody, AI267024 antibody, Agat antibody, O-6-methylguanine-DNA methyltransferase antibody, MGMT antibody, Mgmt antibody

Background: MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation, only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, high MGMT activity was associated with the presence of mutant p53, in a study of oral cancer cell lines. Similarly, MGMT activity was significantly lower in ovarian tumors with wildtype p53 than in tumors with mutant p53, supporting the view that wildtype p53 down-regulates the basal MGMT promoter.

UniProt: P26187, P24528

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus