MGMT antibody (AA 101-207)

Catalog No. ABIN730768

This Rabbit Polyclonal antibody specifically detects MGMT in WB, IHC (p), IHC (fro), IF (cc) and IF (p). It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for MGMT antibody (AA 101-207) (ABIN730768)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-MGMT Antibody

Binding Specificity: AA 101-207

Purpose: MGMT Polyclonal Antibody

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MGMT

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-MGMT antibody (ABIN730768)

Oláh, Kálmán, Tóth, Zvara, Sántha, Ivitz, Janka, Pákáski: "Proteomic Analysis of Cerebrospinal Fluid in Alzheimer's Disease: Wanted Dead or Alive." in: Journal of Alzheimer's disease : JAD, (2014) (PubMed).

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT

Background:

Synonyms: Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase

Background: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Gene ID: 4255

UniProt: P16455

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus