LIF antibody (AA 25-203)
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- Target See all LIF Antibodies
- LIF (Leukemia Inhibitory Factor (LIF))
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Binding Specificity
- AA 25-203
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This LIF antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Leukemia Inhibitory Factor (LIF)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against LIF. It has been selected for its ability to recognize LIF in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Leukemia Inhibitory Factor (LIF) corresdonding to Pro25~Phe203 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product LIF Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
1:200-800 Immunohistochemistry: 5-20 μg/mL
1:20-80 Immunocytochemistry: 5-20 μg/mL
1:20-80 Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- LIF (Leukemia Inhibitory Factor (LIF))
- Alternative Name
- Leukemia Inhibitory Factor (LIF Products)
- Synonyms
- CDF antibody, DIA antibody, HILDA antibody, MLPLI antibody, LIF, interleukin 6 family cytokine antibody, leukemia inhibitory factor antibody, LIF antibody, Lif antibody
- Background
- CDF, D-FACTOR, HILDA, MLPLI, Cholinergic Differentiation Factor, Differentiation-Stimulating Factor, Melanoma-Derived LPL Inhibitor, Emfilermin
- Pathways
- JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
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