EIF3F antibody (AA 114-125)

Catalog No. ABIN214341

Product Details anti-EIF3F Antibody

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Binding Specificity: AA 114-125

Reactivity: Human, Mouse, Rat, Cow, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3F antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Amino acids 114-125 of human eIF3f protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human EIF3F.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:40000), IF, IHC, IHC-P (2.5 μg/mL), IP, WB (1:200 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for EIF3F

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Alternative Name: EIF3S5 / EIF3F (EIF3F Products)

Synonyms: AO090005001590 antibody, Dmel\CG9769 antibody, eIF3-S5 antibody, eif3f antibody, LOC733116 antibody, eIF3f antibody, EIF3S5 antibody, eIF3-p47 antibody, 0610037M02Rik antibody, AA409853 antibody, Eif3s5 antibody, eukaryotic translation initiation factor 3 subunit F antibody, hypothetical protein antibody, CG9769 gene product from transcript CG9769-RA antibody, eukaryotic translation initiation factor 3 subunit 5 antibody, eukaryotic translation initiation factor 3, subunit F antibody, Eukaryotic translation initiation factor 3 subunit F antibody, eif3f antibody, ANI_1_120074 antibody, AOR_1_2780174 antibody, PTRG_03897 antibody, PAAG_00376 antibody, MCYG_08502 antibody, MGYG_08653 antibody, PGTG_04587 antibody, CG9769 antibody, LOC733116 antibody, EIF3F antibody, Eif3f antibody, eif-3.F antibody

Background: Name/Gene ID: EIF3F

Synonyms: EIF3F, Deubiquitinating enzyme eIF3f, EIF-3-epsilon, EIF3 p47, EIF3S5, EIF3-epsilon, EIF3-p47

Gene ID: 8665

UniProt: O00303

Pathways: Ribonucleoprotein Complex Subunit Organization