HIF3A antibody (AA 581-592)

Catalog No. ABIN214599

Product Details anti-HIF3A Antibody

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Binding Specificity: AA 581-592

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIF3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Hif3 a (Hypoxia Inducible Factor).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from mouse HIF3A.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:14000 - 1:80000), IF, IHC, IHC-P (5 μg/mL), WB (1:100 - 1:8000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.***Does not work to detect human protein in Western blot.***

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HIF3A

Target: HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))

Alternative Name: HIF3A / HIF3-Alpha (HIF3A Products)

Synonyms: HIF-3A antibody, IPAS antibody, MOP7 antibody, PASD7 antibody, bHLHe17 antibody, Ipas antibody, NEPAS antibody, Mop7 antibody, HIF3A antibody, hypoxia inducible factor 3 alpha subunit antibody, hypoxia inducible factor 3, alpha subunit antibody, HIF3A antibody, Hif3a antibody

Background: Name/Gene ID: HIF3A

Synonyms: HIF3A, BHLHe17, HIF-3A, IPAS, HIF3-alpha-1, Inhibitory PAS domain protein, PASD7, HIF-3-alpha, HIF3-alpha, Member of PAS protein 7, MOP7

Gene ID: 64344

UniProt: Q9Y2N7

Pathways: Warburg Effect