SPRY4 antibody (AA 306-322)

Catalog No. ABIN214620

Product Details anti-SPRY4 Antibody

Target: SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))

Binding Specificity: AA 306-322

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRY4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Amino acids 306-322 of Human Sprouty-4 protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human SPRY4.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:3000 - 1:12000), IHC, IHC-P (2.5 μg/mL), WB (1:100 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for SPRY4

Target: SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))

Alternative Name: Sprouty 4 / SPRY4 (SPRY4 Products)

Synonyms: SPRY4 antibody, LOC100218545 antibody, HH17 antibody, A030006O18Rik antibody, sprouty4 antibody, sprouty RTK signaling antagonist 4 antibody, sprouty (Drosophila) homolog 4 antibody, sprouty homolog 4 (Drosophila) antibody, Spry4 antibody, SPRY4 antibody, spry4 antibody

Background: Name/Gene ID: SPRY4

Synonyms: SPRY4, Protein sprouty homolog 4, Sprouty 4, Sprouty homolog 4 (Drosophila), Sprouty 4, Spry-4

Gene ID: 81848

UniProt: Q9C004