CDK2 antibody (AA 1-100)

Catalog No. ABIN2172819

Product Details anti-CDK2 Antibody

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Binding Specificity: AA 1-100

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Cow,Pig,Horse,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CDK2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CDK2 antibody (ABIN2172819)

Ghate, Das, Chaudhuri, Panja, Mandal: "Sundew plant, a potential source of anti-inflammatory agents, selectively induces G2/M arrest and apoptosis in MCF-7 cells through upregulation of p53 and Bax/Bcl-2 ratio." in: Cell death discovery, Vol. 2, pp. 15062, (2016) (PubMed).

Chen, Wang, Wang, Huang, Zhang: "STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7." in: Cancer cell international, Vol. 15, pp. 111, (2015) (PubMed).

Ghate, Chaudhuri, Sarkar, Sajem, Panja, Rout, Mandal: "An antioxidant extract of tropical lichen, Parmotrema reticulatum, induces cell cycle arrest and apoptosis in breast carcinoma cell line MCF-7." in: PLoS ONE, Vol. 8, Issue 12, pp. e82293, (2013) (PubMed).

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: CDK2 (CDK2 Products)

Synonyms: p33(CDK2) antibody, A630093N05Rik antibody, wu:fa10c02 antibody, zgc:56598 antibody, zgc:77684 antibody, eg1 antibody, CDKN2 antibody, cdk2L antibody, cyclin dependent kinase 2 antibody, cyclin-dependent kinase 2 antibody, cyclin-dependent kinase 2 S homeolog antibody, CDK2 antibody, Cdk2 antibody, cdk2 antibody, cdk2.S antibody

Background:

Synonyms: CDKN2, p33(CDK2), Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase, CDK2

Background: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq, Jul 2008].

Gene ID: 1017

UniProt: P24941

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA