Rhodopsin antibody
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- Target See all Rhodopsin (RHO) Antibodies
- Rhodopsin (RHO)
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Reactivity
- Human, Rat, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Rhodopsin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- Recognizes a protein of 39kD identified as rat rhodopsin (opsin). Specifically labels the axons and synaptic pedicles of the rods. Species cross-reactivity: human and mouse.
- Purification
- Protein G purified
- Immunogen
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Membrane preparation from adult rat retina. MW of Antigen: 39kD. Cellular Localization: Cytoplasmic.
Type of Immunogen: Membrane extract - Isotype
- IgG1
- Top Product
- Discover our top product RHO Primary Antibody
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- Application Notes
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Approved: IF, IHC, IHC-P, WB (1 - 2 μg/mL)
Usage: Useful in studies of photoreceptor cells. Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Western Blot: 1-2 μg/mL for 2 hours at RT. Immunohistochemistry (Formalin/paraffin): 1:25-1:50 for 60 minutes at RT. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: Brain or retina. - Comment
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Target Species of Antibody: Rat
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 0.2 % BSA, 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Target
- Rhodopsin (RHO)
- Alternative Name
- Rhodopsin / RHO (RHO Products)
- Synonyms
- CSNBAD1 antibody, OPN2 antibody, RP4 antibody, RH1 antibody, Rh antibody, fi06d11 antibody, wu:fi06d11 antibody, zfo2 antibody, zfrho antibody, Noerg1 antibody, Opn2 antibody, Ops antibody, opn2 antibody, rh1 antibody, rp4 antibody, xrho antibody, PIGRHO1 antibody, ops antibody, RDP1 antibody, RHO antibody, rhodopsin antibody, rhodopsin L homeolog antibody, RHO antibody, Rho antibody, rho antibody, rho.L antibody
- Target Type
- Chemical
- Background
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Name/Gene ID: RHO
Subfamily: Opsin
Family: GPCR
Synonyms: RHO, CSNBAD1, OPN2, Rhodopsin, RP4, Opsin 2, rod pigment, Opsin-2 - Gene ID
- 6010
- UniProt
- P08100
- Pathways
- WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction
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