MGMT antibody

Catalog No. ABIN6735110

The Mouse Monoclonal anti-MGMT antibody (ABIN6735110) specifically detects MGMT in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for MGMT antibody (ABIN6735110)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MGMT Antibody

Specificity: Recognizes human MGMT.

Purification: Immunoaffinity purified

Immunogen: Purified recombinant human MGMT protein (~25kD) expressed in E. coli. Cellular Localization: cytoplasmic and nuclear.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), WB (1:500)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1:500. Detects ~25kD in liver and many tumor cells (Molt-4, Raji, HeLa, HT-292, etc). ELISA: 1:10000-1:50000 using 50-100 ng control peptide/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 100 mM Tris,  pH 7.5, 0.2 % BSA, 0.05 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT

Background: Name/Gene ID: MGMT

Synonyms: MGMT

Gene ID: 4255

UniProt: P16455

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus