TNF alpha antibody

Catalog No. ABIN236618

The Mouse Monoclonal anti-TNF alpha antibody is suitable to detect TNF alpha in samples from Human. It has been validated for ELISA, PrA and IHC (f).

Quick Overview for TNF alpha antibody (ABIN236618)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: ELISA, Protein Assay (PrA), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: F6C5

Product Details anti-TNF alpha Antibody

Specificity: Effective in inhibition of the biological activity of recombinant and natural TNF-alpha.

Characteristics: MAb to TNF Alpha, Monoclonal antibody to Human Tumor Necrosis Factor alpha (TNF-alpha)

Purification: Protein A Chromatography. Ascites

Purity: > 90 % pure (SDS-PAGE)

Immunogen: Human recombinant tumor necrosis factor of alpha type

Isotype: IgG1

Application Details

Application Notes: Inhibition of biological activity of TNF-alpha in different biological fluids. Detection of TNF-alpha in two-site immunoassay. Immunohistochemistry using formalin-fixed frozen tissues. Not tested with paraffin-embedded sections. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, 0.1 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor Necrosis Factor alpha (TNF-alpha)

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome