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Tumor Protein P53 (TP53) (pSer392) antibody
| Antigen | Tumor Protein P53 (TP53) |
| Synonyms | p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53 |
| Epitope |
 Alternatives pSer392 |
| Clonality | Monoclonal (FP3-2) |
| Host |
Alternatives Mouse |
| Reactivity |
Alternatives Human |
| Application |
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|
| Catalog no. | ABIN238404 |
| Quantity | 0.1 mg (1.0 mg/ml) (Variants) |
| Price | 290.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Characteristics | Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13 |
| Alternative name | P53 (TP53) |
| Swiss-Prot | P04637 |
| Immunogen | KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P] corresponding to amino acids378-393 representing the C-terminus of Human p53. |
| Cross-Reactivity | Human |
| Isotype | IgG1 (Matching secondary antibodies) |
| Clone | FP3-2 |
| Description | The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation forpyrimidine nukleotides. It is up-regulated in response to these stress signals andstimulated to activate transcription of specific genes, resulting in expression of p21waf1and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such asBcl-2. The structure of p53 comprises N-terminal transactivation domain, centralDNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. Thereare various phosphorylation sites on p53, of which the phosphorylation at Ser15 isimportant for p53 activation and stabilization. |
| Specificity | This p53 antibody FP3. 2 [FPS392] reacts with Human p53 tumour suppressor proteinphosphorylated at CKII site (Ser392). Species Reactivity: Tested: Human, Mouse. Expected from sequence similarity: Rabbit (85%), Canine (85%), Guinea pig (85%), |
Application Details
| Application Notes | Western Blotting: 1 μg/ml. Immunohistochemistry on Paraffin Sections): 10 μg/mlStaining technique: Standard ABC technique (DAB+)Pretreatment: High temperature antigen retrieval (microwave, pressure cooker) in 10 mMcitrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0. Incubation Time: 1 hour at RT or overnight at 4°CPositive Tissue: breast carcinoma with high level of wild-type p53. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. |
| Concentration | 1.0 mg/ml |
| Purification | Aff - Purified |
| Buffer | PBS, pH~7.4 containing 15 mM Sodium Azide as preservative. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Research Area | Transcription Factors, Phospho-specific antibodies |
| Restrictions | For Research Use only |
Alternatives
Alternatives for antigen "Tumor Protein P53 (TP53)", type "Antibodies"
