HLA Class II DR antibody

Antigen: HLA Class II DR

Clonality: Monoclonal (MEM-12)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN238445

Additional Information

Characteristics: Synonyms: HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR

Immunogen: Thymocyte membrane

Cross-Reactivity: Human

Isotype: IgG1  (Matching secondary antibodies)

Clone: MEM-12

Description: HLA DR, like other MHC class II molecules, is a transmembrane glycoprotein composed ofan alpha chain (36 kDa) and a beta chain (27 kDa). It is expressed primarily on antigenpresenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cellsand activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressedproducts of the human class II region. The human MHC class II molecules bindintracellularly processed peptides and present them to T helper cells. They therefore havea critical role in the initiation of the immune response.

Specificity: The antibody MEM-12 recognizes common epitope on human HLA-DR which is dependenton the association of alpha and beta chains. DR is the isotype of human MHC Class IImolecules expressed on antigen-presenting cells (APC, Dendritic cells, B Lymphocytes,Monocytes, Macrophages). Species: Human. Others not tested.

Application Details

Application Notes: Flow Cytometry: Positive Control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Immunoprecipitation: Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Western Blotting (Non-Reducing Conditions): Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Sample preparation: Resuspend approx.50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing SDS-PAGE sample buffer. Do not heat/boil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Major Histocompatibility Complex (MHC)

Restrictions: For Research Use only