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Antibodies
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anti-Integrin beta 2 (ITGB2) (Activation Site) antibody (APC)
Integrin beta 2 (ITGB2) (Activation Site) antibody (APC)
| Antigen |
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| Synonyms |
2E6, LAD, Cd18, Lfa1, MF17, LCAMB, AI528527, CD18, MFI7, LFA-1, MAC-1, CD61, GP3A, GPIIIa, INGRB3, MGC159221, MGC159223, ITGB2, integrin, ITGB3, MGC116306 |
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Epitope
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Activation Site
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| Clonality |
Monoclonal (MEM-148) |
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Host
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Reactivity
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Conjugate
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Application
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| Catalog no. |
ABIN238446 |
| Quantity |
100 Tests |
| Price |
330.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Integrin beta-2, MFI7, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunitbeta, Complement receptor C3 subunit beta
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Alternative name
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CD18 / ITGB2
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Swiss-Prot
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P05107
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Immunogen
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Peripheral blood mononuclear cells.
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Cross-Reactivity
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Human
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Isotype
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IgG1
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Clone
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MEM-148
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Description
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CD18, integrin β2 subunit, forms heterodimers with four types of CD11 molecule toconstitute leukocyte (β2) integrins: αLβ2 (CD11a/CD18, LFA-1), αMβ2 (CD11b/CD18, Mac-1,CR3), αXβ2 (CD11c/CD18) and αDβ2 (CD11d/CD18). In most cases, the response mediatedby the integrin is a composite of the functions of its individual subunits. These integrinsare essential for proper leukocyte migration, mediating intercellular contacts. Absence ofCD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leadsto the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia(Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediatedcytolysis.
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Specificity
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The antibody MEM-148 recognizes the epitope exposed on free human CD18 chainsunassociated with CD11 as well as on high-affinity state of LFA-1. CD18 (integrin β2 subunit,β2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. Negative Species: PorcineSpecies: Human. Others not tested.
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Application Notes
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Suitable for Flow Cytometry analysis of human blood cells using 20 μl reagent / 100 μl ofwhole blood or 10e6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Buffer
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PBS containing 15 mM sodium azide as preservative and 0.2% (w/v)high-grade BSA (Protease free) as stabilizer.Label: Conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.
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Storage
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Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light. Shelf life: One year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "Integrin beta 2 (ITGB2)", type "Antibodies"
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Hosts
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Mouse (45), Rat (26), Rabbit (3)
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Reactivities
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Human (41), Mouse (Murine) (16), Dog (Canine) (11), Rat (Rattus) (8), Pig (Porcine) (4), Cow (Bovine) (2), Rabbit (1)
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Applications
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Flow Cytometry (FACS) (68), Immunoprecipitation (IP) (32), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Western Blotting (WB) (20), Functional Studies (Func) (8), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), ELISA (Detection) (2), Enzyme Immunoassay (EIA) (2), Immunofluorescence (IF) (1)
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Conjugates
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FITC (11), PE (8), Biotin (7), APC (1)
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Alternatives 
