Nodal Homolog (Mouse) (NODAL) antibody

Antigen: Nodal Homolog (Mouse) (NODAL)

Synonyms: MGC138230, NODAL

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Enzyme Immunoassay (EIA)

Catalog no.: ABIN238582

Additional Information

Alternative name: NODAL

Gene ID: 4838, 18119 (mouse)

NCBI Acession: NP_060525.2

Immunogen: Peptide with sequence ECPNPVGEEFH, from the internal region of the protein sequence according to NP_060525.2.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Format: Liquid

Grade: Serum-free

Description: Official Symbol: NODAL

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8000. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant NODAL captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

Concentration: 100µg/200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Hendrix, Seftor, Seftor et al.: "Reprogramming metastatic tumour cells with embryonic microenvironments." in: Nature reviews. Cancer, Vol. 7, Issue 4, pp. 246-55, 2007 (PubMed).