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NCEH1 antibody (Neutral Cholesterol Ester Hydrolase 1) (C-Term)

Details for Product anti-NCEH1 Antibody No. ABIN238671, Supplier: Log in to see
Antigen
  • AADACL1
  • nceh
  • aadacl1
  • MGC79479
  • NCEH
  • Aadacl1
  • B230106I24Rik
  • CPO-BP
  • Nceh
  • RGD1311104
Epitope
C-Term
11
3
1
Reactivity
Human, Mouse (Murine), Dog (Canine)
23
13
7
2
Host
Goat
13
12
Clonality
Polyclonal
Conjugate
This NCEH1 antibody is un-conjugated
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
19
17
2
2
1
Supplier
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Immunogen Peptide with sequence C-RTRNSYIKWLDQN, from the C Terminus of the protein sequence according to NP_065843.3.
Blocking Peptide Blocking peptide for this product available: ABIN369837
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name AADACL1 (NCEH1 Antibody Abstract)
Background Synonyms: AADACL1, arylacetamide deacetylase-like 1
Gene ID 57552, 320024
NCBI Accession NP_065843
Application Notes ELISA: antibody detection limit dilution 1:1000.
Western blot: Preliminary experiments gave an approx 26-28 kDa band in Human Brain (Amygdala, Cerebellum, Hippocampus) lysates after 1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 49.1 kDa according to NP_065843.3. The 26-28 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Chiang, Niessen, Saghatelian et al.: "An enzyme that regulates ether lipid signaling pathways in cancer annotated by multidimensional profiling." in: Chemistry & biology, Vol. 13, Issue 10, pp. 1041-50, 2006 (PubMed).