HSP40-4 antibody
-
- Target
- HSP40-4
- Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- Un-conjugated
-
Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Purification
- Affinity purification
- Immunogen
- Recombinant protein of human DNAJA1
- Isotype
- IgG
-
-
- Application Notes
- WB 1:500-1:2000, IHC 1:100-1:300
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- PBS with 0.05 % sodium azide and 50 % glycerol, PH7.4
- Preservative
- Sodium azide
- Handling Advice
- Avoid freeze / thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze / thaw cycles.
-
- Target
- HSP40-4
- Background
- DnaJ-like proteins interact with HSP 70 Molecular chaperones and function to facilitate protein folding and mitochondrial protein import. HSP 40-4, also known as HDJ2, is the human DnaJ homolog that functions as a co-chaperone with a cysteine-rich zinc finger domain. The cellular redox enzyme thioredoxin interacts with HSP 40-4, and oxidation and reduction reversibly regulate HSP 40-4 function in response to the changing redox states of the cell. The zinc finger domain of HSP 40-4 may act as a redox sensor of chaperone-mediated protein-folding machinery, since HSP 40-4 inactivation leads to the oxidation of cysteine thiols and a simultaneous release of coordinated zinc. Loss of the HSP 40-4 protein may be linked to severe defects in spermatogenesis that involve aberrant androgen signaling.
- Molecular Weight
- Calculated MW: 45 kDa
-