GFP antibody

Catalog No. ABIN243758

The Rabbit Polyclonal anti-GFP antibody is suitable to detect GFP in samples from Aequorea victoria. It has been validated for WB, IHC and IHC (p).

Quick Overview for GFP antibody (ABIN243758)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GFP Antibody

Specificity: Detects GFP-tagged proteins exogenously expressed in cells. Please note that the GFP tag adds approximately 27kD to the molecular weight of the fusion protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic GFP peptide (KLH-coupled).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (1:800), WB (1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:1000. Immunohistochemistry: 1:800. Useful for paraffin-embedded transfected cells only. Antigen retrieval by heating in citrate /TBST buffer is required.

Comment:

Target Species of Antibody: Aequorea victoria

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Name/Gene ID: GFP

Synonyms: GFP

UniProt: P42212