HLTF antibody

Catalog No. ABIN2460973

Product Details anti-HLTF Antibody

Target: HLTF (Helicase-Like Transcription Factor (HLTF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLTF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMARCA3.

Application Details

Application Notes: SMARCA3 antibody can be used for detection of SMARCA3 by ELISA at 1:312500. SMARCA3 antibody can be used for detection of SMARCA3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store SMARCA3 antibody at -20 °C.

Target Details for HLTF

Target: HLTF (Helicase-Like Transcription Factor (HLTF))

Alternative Name: SMARCA3 (HLTF Products)

Synonyms: smarca3 antibody, wu:fc33f07 antibody, HLTF antibody, SMARCA3 antibody, MGC131155 antibody, HIP116 antibody, HIP116A antibody, HLTF1 antibody, RNF80 antibody, SNF2L3 antibody, ZBU1 antibody, RUSH antibody, AF010600 antibody, BC057116 antibody, P113 antibody, Smarca3 antibody, Snf2l3 antibody, helicase like transcription factor antibody, helicase-like transcription factor antibody, helicase-like transcription factor L homeolog antibody, HLTF antibody, hltf antibody, hltf.L antibody, Hltf antibody

Background: SMARCA3 (HLTF) is a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA3 contains a RING finger DNA binding motif. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform which is truncated at the N-terminus as compared to the full-length protein.

Molecular Weight: 114 kDa, 114 kDa

Gene ID: 6596

NCBI Accession: NP_620636

UniProt: Q14527