RNF14 antibody
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- Target See all RNF14 Antibodies
- RNF14 (Ring Finger Protein 14 (RNF14))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RNF14 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purification
- Antibody is purified by protein A chromatography method.
- Immunogen
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF14.
- Top Product
- Discover our top product RNF14 Primary Antibody
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- Application Notes
- RNF14 antibody can be used for detection of RNF14 by ELISA at 1:312500. RNF14 antibody can be used for detection of RNF14 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
- Handling Advice
- As with any antibody avoid repeat freeze-thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store RNF14 antibody at -20 °C.
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- Target
- RNF14 (Ring Finger Protein 14 (RNF14))
- Alternative Name
- RNF14 (RNF14 Products)
- Synonyms
- ARA54 antibody, HFB30 antibody, TRIAD2 antibody, 2310075C09Rik antibody, 2610005D23Rik antibody, AA986456 antibody, AU041447 antibody, D18Ertd188e antibody, D7Bwg0165e antibody, Triad2 antibody, ring finger protein 14 antibody, RNF14 antibody, Rnf14 antibody
- Background
- RNF14 contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins.The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.
- Molecular Weight
- 52 kDa, 54 kDa, 40 kDa, 54 kDa, 54 kDa
- Gene ID
- 9604
- NCBI Accession
- NP_899648
- UniProt
- Q9UBS8
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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