MRPS12 antibody

Catalog No. ABIN2462968

Product Details anti-MRPS12 Antibody

Target: MRPS12 (Mitochondrial Ribosomal Protein S12 (MRPS12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPS12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MRPS12.

Application Details

Application Notes: MRPS12 antibody can be used for detection of MRPS12 by ELISA at 1:1562500. MRPS12 antibody can be used for detection of MRPS12 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MRPS12 antibody at -20 °C.

Target Details for MRPS12

Target: MRPS12 (Mitochondrial Ribosomal Protein S12 (MRPS12))

Alternative Name: MRPS12 (MRPS12 Products)

Synonyms: MPR-S12 antibody, MT-RPS12 antibody, RPMS12 antibody, RPS12 antibody, RPSM12 antibody, AI327385 antibody, Rpms12 antibody, rps12 antibody, CG7925 antibody, Dmel\\CG7925 antibody, EG:BACH59J11.1 antibody, MRP-S12 antibody, MRPS12 antibody, S12 antibody, Tko antibody, l(1)3Ab antibody, l(1)tko antibody, mRpS12 antibody, mt-rps12 antibody, MGC64304 antibody, GB10531 antibody, mitochondrial ribosomal protein S12 antibody, technical knockout antibody, mitochondrial ribosomal protein S12 L homeolog antibody, 40S ribosomal protein S12, mitochondrial antibody, putative mitochondrial 37S ribosomal protein MRPS12 antibody, fragile site, aphidicolin type, common, fra(6)(p22.2) antibody, MRPS12 antibody, Mrps12 antibody, tko antibody, mrps12.L antibody, LOC409723 antibody, mRpS12 antibody, mrps12 antibody, CAALFM_C106070WA antibody, mrpS12 antibody, FRA6C antibody

Background: Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPS12 is the 28S subunit protein that belongs to the ribosomal protein S12P family. The protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics.Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene, all three variants encode the same protein.

Molecular Weight: 12 kDa

Gene ID: 6183

NCBI Accession: NP_066930

UniProt: O15235

Pathways: Sensory Perception of Sound