Western Blotting (WB), Immunoprecipitation (IP), ELISA
Purification
TRPC7 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TRPC7 antibody was raised against a 13 amino acid synthetic peptide near the internal region of TRPC7.
TRPC7
Reactivity: Human
WB, IHC, IP, IF, ICC, AA
Host: Mouse
Monoclonal
N64A-36 (Formerly S64A-36)
Atto 594
Application Notes
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 20 kDa band in lysates of cell lines NIH3T3 and KELLY after 1 μg/mL antibody staining.6 kDa according to NP_065122.1. The 20 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.