ANGPT1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ANGPT1 antibody was raised against a amino acid synthetic peptide near the internal region of ANGPT1.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: In transfected HEK293 transiently expressing full-length human aNGPT1 (myc and DYKDDDDK tagged), a band of approximately 48 kDa was observed. No bands were observed in mock-transfected HEK293 and the 48 kDa plus a 75 kDa band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.