This antibody is expected to recognize isoform 1 (NP_000396.2) only.
Purification
GM2A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
GM2A antibody was raised against an 11 amino acid synthetic peptide near the internal region of GM2A (aa 179-189).
GM2A
Reactivity: Human
WB
Host: Mouse
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:4000. Western Blot: In transfected HEK293 transiently expressing full-length human GM2A (myc and DYKDDDDK tagged), a band of approximately 28 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.