This antibody is expected to recognize both reported isoforms.
Purification
AXIN1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
AXIN1 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of AXIN1.
ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 150 kDa and 22-24 kDa in human hippocampus lysates after 0.1 μg/mL antibody staining.6 kDa according to NP_003493.1 and 91.7 kDa according to NP_851393.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Immunohistochemistry: In paraffin embedded human adrenal gland shows membranous and fibrous staniing in and around secretory cells of the zona fasciculata in the cortex. Recommended concentration, 3-5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.