Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
SH3BP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SH3BP1 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of SH3BP1.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 70 kDa band observed in human lung and kidney lysates (predicted MW of 71 kDa according to NP_061830). Recommended for use at 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration, 0.3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.