This antibody is expected to recognise both reported isoforms (NP_001094345.1, NP_689811.2), which are identical.
Purification
MARCH10 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
MARCH10 antibody was raised against an 11 amino acid synthetic peptide near the internal region of 40978.
MARCH10
Reactivity: Human, Rat, Mouse
WB, IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 594
Application Notes
ELISA: Antibody detection limit dilution 1:8000. Approximately 90 kDa band observed in rat testis lysates (calculated MW of 90.5 kDa according to human NP_689811.2 and of 87.9 kDa according to mouse NP_766156.2). Recommended concentration: 0.3-1 μg/mL. an additional band of unknown identity was also consistently observed at 37 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
MARCH10
(Membrane-Associated Ring Finger (C3HC4) 10, E3 Ubiquitin Protein Ligase (MARCH10))