CENPF antibody (AA 1759-2093)

Catalog No. ABIN2619121

Product Details anti-CENPF Antibody

Target: CENPF (Centromere Protein F (CENPF))

Binding Specificity: AA 1759-2093

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CENPF antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: IHC-plus™

Specificity: Reacts with human Mitosin.

Purification: Protein G purified

Immunogen: Recombinant human CENPF.

Type of Immunogen: Recombinant protein

Clone: 14C10 1D8

Isotype: IgG2a

Application Details

Application Notes: Approved: IF, IHC, IHC-Fr, IHC-P (10 μg/mL), IP, WB (1:500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS buffer ,  pH 7.2

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CENPF

Target: CENPF (Centromere Protein F (CENPF))

Alternative Name: CENPF / CENP-F (CENPF Products)

Synonyms: LOC100222985 antibody, 6530404A22Rik antibody, AI325968 antibody, CENF antibody, Lek1 antibody, PRO1779 antibody, mitosin antibody, im:7140452 antibody, im:7144238 antibody, im:7154719 antibody, im:7156761 antibody, si:ch211-152h14.2 antibody, hcp-1 antibody, CMF1 antibody, centromere protein F antibody, CENPF antibody, Cenpf antibody, cenpf antibody

Background: Name/Gene ID: CENPF

Synonyms: CENPF, AH antigen, Centromere protein F, CENF, CENP-F, Kinetochore protein CENPF, Mitosin, CENP-F kinetochore protein, Hcp-1, PRO1779

Gene ID: 1063

Pathways: Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection