HLA-DR antibody (FITC)

Catalog No. ABIN264299

The Mouse Monoclonal anti-HLA-DR antibody is suitable to detect HLA-DR in samples from Human and Monkey. It has been validated for FACS.

Quick Overview for HLA-DR antibody (FITC) (ABIN264299)

Target: HLA-DR

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DR antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 423L

Product Details anti-HLA-DR Antibody (FITC)

Characteristics: Synonyms: HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry. Monitoring of B cells in peripheral blood. Analysis of B subsets. Study of B cell activation. Study of B cell neoplasms.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. See instrument manufacturers instructions for Lysed Whole Blood andImmunofluorescence analysis with a flow cytometer or microscope.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for HLA-DR

Target: HLA-DR

Alternative Name: HLA Class II DR

Background: Identification of human B cell and T cell subsets associated with approximately 10 % of peripheral blood lymphocytes 28-34,000 M.W. surface antigen, also low density on monocytes and macrophages.Synonyms: HLA class II histocompatibility antigen DR, HLA-DR, MHC class II antigen DR

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response