Flow Cytometry. Monitoring of Mucosa-associated T Lymphocytes. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol
PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze. WHOLE BLOOD:
Restrictions
For Research Use only
Buffer
PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! Shelf life: One year from despatch.
Expiry Date
12 months
Target
CD103 (ITGAE)
(Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))
The CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cells and on subset of TGF beta -1 cells. This human intra epithelial lymphocyte marker is the E integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood ymphocytes. CD103 is expressed by 0.5 to 2 % of resting lymphocytes in Peripheral blood and lymphoid organs.Synonyms: HML-1 antigen, ITGAE, Integrin alpha-E, Integrin alpha-IEL, Mucosal lymphocyte 1 antigen