LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (HMG Binding Domain)

Details for Product anti-LEF1 Antibody No. ABIN264381, Supplier: Log in to see
Antigen
  • LEF1
  • 3000002B05
  • AI451430
  • Lef-1
  • LEF-1
  • TCF10
  • TCF1ALPHA
  • TCF7L3
  • lymphoid enhancer-binding factor 1
  • lymphocyte enhancer binding factor 1
  • lymphoid enhancer binding factor 1
  • lymphoid enhancer factor XLEF-1B
  • LEF1
  • LOC100352691
  • lef1
  • Lef1
Alternatives
anti-Human LEF1 antibody for Enzyme Immunoassay
Epitope
HMG Binding Domain
15
13
13
9
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6
6
6
4
2
2
2
1
1
1
1
1
1
1
1
1
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1
Reactivity
Human, Mouse (Murine)
128
41
32
11
7
7
5
5
4
4
4
4
3
2
1
1
Host
Mouse
57
51
16
5
Clonality (Clone)
Monoclonal ()
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunofluorescence (IF), Western Blotting (WB)
115
67
21
17
10
7
5
3
2
2
2
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Full-length recombinant LEF-1 protein. Genename: LEF1
Clone REMB6
Isotype IgG1
Specificity REMB6 recognizes the High Mobility Group DNA domain of LEF-1.
Characteristics Synonyms: Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha,TCF1-alpha
Purification Purified
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Lymphoid Enhancer Factor-1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of polypeptides ranging in size between 55-57 kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms.Synonyms: LEF-1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha
Gene ID 51176
UniProt Q9UJU2
Research Area Transcription Factors, Wnt, Lineage Markers, Wnt Signaling
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding
Application Notes Western Blot: 1-5 μg/mLImmunofluorescence on Paraformaldehyde-Fixed Cells: 1 μg/mL (1/1000). Positive Control: Jurkat cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS, 0.08 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (HMG Binding Domain) antibody (ABIN264381) anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (HMG Binding Domain) antibody
Immunofluorescence (IF) image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (HMG Binding Domain) antibody (ABIN264381) anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (HMG Binding Domain) antibody (Image 2)
Background publications Hsu, Galceran, Grosschedl: "Modulation of transcriptional regulation by LEF-1 in response to Wnt-1 signaling and association with beta-catenin." in: Molecular and cellular biology, Vol. 18, Issue 8, pp. 4807-18, 1998 (PubMed).

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