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Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase)

Details for Product anti-CASP3 Antibody No. ABIN264404, Supplier: Log in to see
Antigen
  • A830040C14Rik
  • AC-3
  • Apopain
  • Cas3
  • Casp 3
  • Casp3
  • caspase-3
  • Caspase-3
  • caspase 3
  • CC3
  • CG7788
  • CG14902
  • CPP32
  • CPP32B
  • crice
  • Decay
  • DECAY
  • Dmel\\CG7788
  • Dmel\\CG14902
  • drice
  • drIce
  • DrIce
  • DrICE
  • drICE
  • Drice
  • DRICE
  • Drosophila executioner caspase related to Apopain/Yama
  • ICE
  • ice
  • Lice
  • mldy
  • SCA-1
  • xcpp32
  • Yama
Alternatives
anti-Human Caspase 3 antibody for Western Blotting
Reactivity
Human
553
244
197
33
31
23
22
19
17
16
15
7
7
7
6
4
2
1
Host
Mouse
462
146
17
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This Caspase 3 antibody is un-conjugated
28
23
21
14
9
9
9
6
6
4
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
1
1
Application
Western Blotting (WB)
458
234
202
121
94
69
58
52
48
33
26
12
10
7
5
3
2
2
2
2
2
2
2
1
1
1
1
Supplier
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Supplier Product No.
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Immunogen Hybridoma produced by the fusion of splenocytes from mice immunized with recombinanthuman Caspase-3 protein and mouse myeloma cells. Genename: CASP3
Clone AM1-4
Isotype IgG
Specificity This antibody Detects human Caspase-3.
Characteristics Synonyms: CASP-3, CASP3, CPP32, CPP-32, Yama protein, Apopain, SCA-1, SCA1, Cysteine proteaseCPP32, SREBP cleavage activity 1
Purification Purified
Alternative Name Caspase-3 (CASP3 Antibody Abstract)
Background Caspase-3 along with caspase 7 and 6 form the group of effector caspases that are responsible for the cleavage of multiple substrates including the cytokeratins, PARP, alpha fodrin, NuMA and others. Caspase-7 occurs in three varient forms.Caspase-3-like activities are required for Fas-mediated apoptosis. However, the role of caspase-1 and caspase-3 in mediating Fas-induced cell death is not clear. Although wild-type, caspase-1(-/-), and caspase-3(-/-) hepatocytes were killed at a similar rate when cocultured with FasL expressing NIH 3T3 cells, caspase-3(-/-) hepatocytes displayed drastically different morphological changes as well as significantly delayed DNA fragmentation. For both wild-type and caspase-1 (-/-) apoptotic hepatocytes, typical apoptotic features such as cytoplasmic blebbing and nuclear fragmentation are seen within 6 hr, but neither event was observed for caspase-3(-/-) hepatocytes. In thymocytes apoptotic caspase-3 (-/-) thymocytes exhibit similar abnormal morphological changes and delayed DNA fragmentation observed in hepatocytes. Cleavage of various caspase substrates implicates apoptotic events, including gelsolin, fodrin, laminB, and DFF45/ICAD are delayed or absent. The altered cleavage of these key substrates is likely responsible for the aberrant apoptosis observed in both hepatocytes and thymocytes deficient in caspase-3.Synonyms: Apopain, CASP-3, CASP3, CPP-32, CPP32, Cysteine protease CPP32, SCA-1, SCA1, SREBP cleavage activity 1, Yama protein
Gene ID 836
UniProt P42574
Research Area Apoptosis/Necrosis, Autophagy
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.08 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months
Background publications Slee, Harte, Kluck, Wolf, Casiano, Newmeyer, Wang, Reed, Nicholson, Alnemri, Green, Martin: "Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner." in: The Journal of cell biology, Vol. 144, Issue 2, pp. 281-92, 1999 (PubMed).

Ueda, Nakamura, Masutani, Sasada, Yonehara, Takabayashi, Yamaoka, Yodoi: "Redox regulation of caspase-3(-like) protease activity: regulatory roles of thioredoxin and cytochrome c." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 12, pp. 6689-95, 1999 (PubMed).

Samali, Cai, Zhivotovsky, Jones, Orrenius: "Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells." in: The EMBO journal, Vol. 18, Issue 8, pp. 2040-8, 1999 (PubMed).

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).