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Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase)

Details for Product anti-CASP8 Antibody No. ABIN264408, Supplier: Login to see
Antigen
  • casp8-A
  • xCaspase-8
  • casp8
  • CASP8
  • CASP-8
  • FLICE
  • MACH
  • Mch5
  • ALPS2B
  • CAP4
  • Casp-8
  • MCH5
  • caspase-8
  • zgc:92075
Reactivity
Human
304
95
65
18
13
4
1
1
1
Host
Mouse
273
67
11
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Caspase 8 antibody is un-conjugated
16
11
6
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
291
113
72
50
45
27
19
18
11
11
8
5
2
2
1
1
1
1
1
Supplier
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Immunogen Hybridoma produced by the fusion of splenocytes from mice immunized with recombinanthuman capase-8 protein and mouse myeloma cells. Genename: CASP8
Clone FLICE 3
Isotype IgG
Specificity This antibody detects proenzyme form of human Caspase-8 by Western blot analysis as well as one of the cleavage products.
Characteristics Synonyms: CASP-8, CASP8, MCH5, CAP4, ICE-like apoptotic protease 5, MORT1-associated CED-3homolog, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, Apoptotic cysteineprotease, Apoptotic protease Mch-5
Purification Purified
Alternative Name Caspase-8 (CASP8 Antibody Abstract)
Background Caspases are key effectors of programmed cell death. They are synthesized as inactive proenzymes which are activated by cleavage at a specific aspartate residue to form two subunits. These subunits are normally linked together by a linker which may be involved in the regulation of the different caspases. Caspase-8 is a key enzyme in the apoptosis pathway. Caspase-8 contains two N-terminal region death effector domains which are removed to activate the enzyme along with cleavage into the two subunits. These subunits then for the active protease which is capable of cleaving caspase-3, -6 and -7. These caspases are then capable of cleaving other cellular substrates such as PARP and DFF, which in turn induce apoptosis.Synonyms: Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, CASP-8, CASP8, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MCH5, MORT1-associated CED-3 homolog
Gene ID 841
UniProt Q14790
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling
Application Notes Western Blot. Positive Control: MCF-7 cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.08 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Caspase 8 antibody (Caspase 8, Apoptosis-Related Cysteine Peptidase) (ABIN264408) anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) antibody
Background publications Green: "Apoptotic pathways: the roads to ruin." in: Cell, Vol. 94, Issue 6, pp. 695-8, 1998 (PubMed).

Martin, Siegel, Zheng et al.: "Membrane oligomerization and cleavage activates the caspase-8 (FLICE/MACHalpha1) death signal." in: The Journal of biological chemistry, Vol. 273, Issue 8, pp. 4345-9, 1998 (PubMed).

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).

Hu, Vincenz, Ni et al.: "I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 28, pp. 17255-7, 1997 (PubMed).