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Phosphothreonine antibody

Details for Product No. ABIN264877, Supplier: Log in to see
Antigen
Reactivity
Chemical
78
3
1
Host
Rabbit
62
20
Clonality
Polyclonal
Conjugate
Un-conjugated
9
8
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
76
64
52
19
17
13
11
3
1
1
1
1
Supplier
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Immunogen Phosphothreonine conjugated to KLH
Isotype IgG
Purification Affinity Chromatography
Target Type Chemical
Background Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Application Notes ELISA: 0.5 μg/mL (Ref.5)Western blot: 2 μg/mL (Ref.5)Immunoprecipitation: 10 μg/mg sample.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % Sodium Azide, 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-Phosphothreonine antibody (ABIN264877) anti-Phosphothreonine antibody
Background publications Goto, Kiyono, Tomono et al.: "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition." in: Nature cell biology, Vol. 8, Issue 2, pp. 180-7, 2006 (PubMed).

Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).