Phosphothreonine antibody

Catalog No. ABIN5585637

Product Details anti-Phosphothreonine Antibody

Target: Phosphothreonine

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Phosphothreonine antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose: Recombinant rabbit monoclonal antibody raised against phosphothreonine.

Specificity: This antibody reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.

Immunogen: Original antibody is raised against a mixture of phosphothreonine conjugated with BSA and phosphothreonine containing peptide.

Clone: RM102

Isotype: IgG

Application Details

Application Notes: ELISA
Flow Cytometry
Immunocytochemistry (1:100-1:500)
Immunohistochemistry (1:100-1:500)
Immunoprecipitation (1:100-1:500)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS (50 % glycerol, 1 % BSA, 0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Target Details for Phosphothreonine

Target: Phosphothreonine

Abstract: Phosphothreonine Products

Target Type: Amino Acid