E-cadherin antibody (Arg3)

Catalog No. ABIN265314

The Rabbit Polyclonal anti-E-cadherin antibody (ABIN265314) specifically detects E-cadherin in WB, IF, IHC (p) and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for E-cadherin antibody (Arg3) (ABIN265314)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-E-cadherin Antibody

Binding Specificity: Arg3

Specificity: This antibody detects endogenous levels of ARC protein. (region surrounding Glu194)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1/40000-1/60000. Western blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: ARC / .1

Background: Growth factor stimulation has been shown to induce the expression of immediate early genes in non-neuronal cells, which encode a variety of molecules that are potentially involved in long-term cellular responses. Similar responses induced by neurotransmitter stimulation have also been seen in neuronal cells and evidence suggests that protein synthesis is required for long-term synaptic plasticity. Arc (for activity-regulated cytoskeleton-associated protein) is a growth factor and immediate early gene that is enriched in brain. Arc mRNA and protein levels are induced by neuronal activity, which is necessary to stimulate neuroplasticity, indicating a potential role for Arc in activitydependent changes in dendrite function. Arc expression has been detected in neuronal cell bodies and dendrites in the hippocampus, amygdala, hypothalamus, striatum and cortex. Arc has been shown to localize to the cytoskeleton of neuronal cells and appears to co-localize with F-Actin, although it may associate with an Actin-associated protein rather than directly with F-Actin. It has been shown that cocaine-stimulated neuronal activity results in increased Arc mRNA levels in striatum.Synonyms: Activity-regulated cytoskeleton-associated protein, Activity-regulated gene 3.1 protein homolog, KIAA0278

Molecular Weight: approx. 24 or 28 kDa

Gene ID: 23237

NCBI Accession: NP_056008

UniProt: Q7LC44

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation