PRAF2 antibody

Catalog No. ABIN265488

Product Details anti-PRAF2 Antibody

Target: PRAF2 (PRA1 Domain Family, Member 2 (PRAF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of JM4/PRAF2 protein. (region surrounding Leu166)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PRAF2

Target: PRAF2 (PRA1 Domain Family, Member 2 (PRAF2))

Alternative Name: JM4 / PRAF2 (PRAF2 Products)

Synonyms: AA986329 antibody, C78013 antibody, DXImx39e antibody, JM4 antibody, Sfc20 antibody, PRA1 domain family member 2 antibody, PRA1 domain family 2 antibody, PRAF2 antibody, Praf2 antibody

Background: JM4 (Jena-Muenchen 4), also known as PRAF2 (PRA1 domain family, member 2), is a 178 amino acid endosomal multi-pass membrane protein involved in vesicular trafficking and Endoplasmic reticulum/Golgi transport. As a member of the PRA1 family, JM4 contains four putative transmembrane (TM) domains, interacts with the CC chemokine receptor 5 (CCR5) and colocalizes with Calnexin in the ER and mannose 6-phosphate receptor (CD-MPR) in the Golgi apparatus. While ubiquitously expressed, JM4 has been found at high levels in small intestine, lung, pancreas, spleen, Purkinje cells of the cerebellum and in neuronal cells of the hippocampus, cerebral cortex and lateral ventricles of the brain. JM4 plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis and has been implicated in human cancer.Synonyms: PRA1 family protein 2

Molecular Weight: approx. 20 kDa

Gene ID: 11230

NCBI Accession: NP_009144

UniProt: O60831

Pathways: Dicarboxylic Acid Transport