CD8 antibody (CD8a Molecule) (PerCP)

Details for Product anti-CD8A Antibody No. ABIN2659884, Supplier: Log in to see
Antigen
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Alternatives
anti-Human CD8 antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Rat (Rattus)
618
525
126
92
44
26
22
19
13
13
13
12
9
5
5
5
4
4
4
2
2
1
1
Host
Mouse
790
488
198
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD8 antibody is conjugated to PerCP
151
149
93
60
47
41
40
36
31
29
24
23
23
23
23
22
20
19
19
18
15
13
11
11
11
11
10
9
9
8
7
7
7
4
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA (Capture), ELISpot (Capture) (ELISPOT (Capture)), Neutralization (Neut), Western Blotting (WB)
1206
446
365
257
248
235
200
185
182
39
35
20
16
13
11
11
9
6
6
6
6
3
2
2
2
2
2
2
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Clone OX-8
Isotype IgG1 kappa
Purification The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
Alternative Name CD8a (CD8A Antibody Abstract)
Background CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.
Supplier Images
Flow Cytometry (FACS) image for anti-CD8a Molecule (CD8A) antibody (PerCP) (ABIN2659884) anti-CD8a Molecule (CD8A) antibody (PerCP)
Background publications Mitnacht, Bischof, Torres-Nagel, Hünig: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, 1998 (PubMed).

Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, 1997 (PubMed).

Wallgren, Karlsson-Parra, Korsgren: "The main infiltrating cell in xenograft rejection is a CD4+ macrophage and not a T lymphocyte." in: Transplantation, Vol. 60, Issue 6, pp. 594-601, 1995 (PubMed).

Torres-Nagel, Kraus, Brown, Tiefenthaler, Mitnacht, Williams, Hünig: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, 1992 (PubMed).

Johnson, Gagnon, Barclay, Williams: "Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes." in: The EMBO journal, Vol. 4, Issue 10, pp. 2539-45, 1985 (PubMed).

Thomas, Green: "Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens." in: European journal of immunology, Vol. 13, Issue 10, pp. 855-8, 1984 (PubMed).

Mason, Arthur, Dallman, Green, Spickett, Thomas: "Functions of rat T-lymphocyte subsets isolated by means of monoclonal antibodies." in: Immunological reviews, Vol. 74, pp. 57-82, 1983 (PubMed).

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981 (PubMed).

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