HLA-A antibody (Major Histocompatibility Complex, Class I, A) (Pacific Blue)

Details for Product anti-HLA-A Antibody No. ABIN2662341, Supplier: Log in to see
Antigen
  • HLAA
  • major histocompatibility complex, class I, A
  • HLA-A
Alternatives
anti-Human HLA-A antibody for Cytometry by Time of Flight
Reactivity
Human
168
23
9
6
4
4
Host
Mouse
154
25
5
Clonality (Clone)
Monoclonal ()
Conjugate
This HLA-A antibody is conjugated to Pacific Blue
11
9
5
4
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
122
56
45
45
28
18
14
10
5
3
2
2
2
2
1
Options
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Available images

Clone W6-32
Isotype IgG2a kappa
Cross-Reactivity Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey, Cow (Bovine), Cat (Feline)
Purification The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Alternative Name HLA-A (HLA-A Antibody Abstract)
Background MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells. They play an important role in cell-mediated immune responses and tumor surveillance.
Research Area Major Histocompatibility Complex (MHC), Immunology
Pathways TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.
Supplier Images
Flow Cytometry (FACS) image for anti-HLA-A antibody (Major Histocompatibility Complex, Class I, A)  (Pacific Blue) (ABIN2662341) anti-Major Histocompatibility Complex, Class I, A (HLA-A) antibody (Pacific Blue)
Background publications Ayyoub, Taub, Keohan, Hesdorffer, Metthez, Memeo, Mansukhani, Hibshoosh, Hesdorffer, Valmori: "The frequent expression of cancer/testis antigens provides opportunities for immunotherapeutic targeting of sarcoma." in: Cancer immunity, Vol. 4, pp. 7, 2004 (PubMed).

Tran, Ivanyi, Hilgert, Brdicka, Pla, Breur, Flieger, Ivasková, Horejsí: "The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex." in: Immunogenetics, Vol. 53, Issue 6, pp. 440-6, 2001 (PubMed).

Fayen, Huang, Ferrone, Tykocinski: "Negative signaling by anti-HLA class I antibodies is dependent upon two triggering events." in: International immunology, Vol. 10, Issue 9, pp. 1347-58, 1998 (PubMed).

De Felice, Turco, Costanzo, Corbo, Ferrone, Venuta: "Inhibition by anti-HLA class I mAb of IL-2 and IL-2 receptor synthesis in lymphocytes stimulated with PHA-P." in: Cellular immunology, Vol. 126, Issue 2, pp. 420-7, 1990 (PubMed).

Darrow, Slingluff, Seigler: "The role of HLA class I antigens in recognition of melanoma cells by tumor-specific cytotoxic T lymphocytes. Evidence for shared tumor antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 9, pp. 3329-35, 1989 (PubMed).

Geppert, Wacholtz, Patel, Lightfoot, Lipsky: "Activation of human T cell clones and Jurkat cells by cross-linking class I MHC molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 11, pp. 3763-72, 1989 (PubMed).

Turco, de Felice, Corbo, Giarrusso, Yang, Ferrone, Venuta: "Enhancing effect of anti-HLA class I monoclonal antibodies on T cell proliferation induced via CD2 molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 7, pp. 2275-81, 1988 (PubMed).

Stern, Beresford, Friedman, Stevens, Risk, Johnson: "Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 4, pp. 1088-91, 1987 (PubMed).

Barbatis, Woods, Morton, Fleming, McMichael, McGee: "Immunohistochemical analysis of HLA (A, B, C) antigens in liver disease using a monoclonal antibody." in: Gut, Vol. 22, Issue 12, pp. 985-91, 1982 (PubMed).