CLEC9A antibody (PE)

Catalog No. ABIN2662628

Product Details anti-CLEC9A Antibody (PE)

Target: CLEC9A (C-Type Lectin Domain Family 9, Member A (CLEC9A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CLEC9A antibody is conjugated to PE

Application: Intracellular Flow Cytometry (ICFC)

Purification: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Clone: 8F9

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for CLEC9A

Target: CLEC9A (C-Type Lectin Domain Family 9, Member A (CLEC9A))

Alternative Name: CD370 (CLEC9A Products)

Synonyms: 9830005G06Rik antibody, DNGR-1 antibody, DNGR1 antibody, UNQ9341 antibody, RGD1562513 antibody, C-type lectin domain family 9, member a antibody, C-type lectin domain containing 9A antibody, C-type lectin domain family 9, member A antibody, Clec9a antibody, CLEC9A antibody

Background: CD370 (CLEC9A), also known as DNGR1, is a type II transmembrane protein consisting of a single extracellular C-type lectin domain (CTLD) that is connected to the transmembrane domain by a stalk region, and an intracellular cytoplasmic tail with immunoreceptor tyrosine-based activation motif (ITAM). In humans, CLEC9A is strictly expressed on BDCA3-positive conventional dendritic cells (cDC) and CD16- monocytes. CLEC9A recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.