IL-27 antibody

Catalog No. ABIN2666012

Product Details anti-IL-27 Antibody

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func), Immunofluorescence (IF), Intracellular Flow Cytometry (ICFC)

Brand: Ultra-LEAF™

Purification: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level:

Endotoxin level is <0.01 EU/μg of the protein (<0.001 ng/μg of the protein) as determined by the LAL test.

Clone: MM27-7B1

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Preservative: Azide free

Handling Advice: This Ultra-LEAF™ solution contains no preservative, handle under aseptic conditions

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for IL-27

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Alternative Name: IL-27 p28 (IL27 Products)

Synonyms: IL-27 antibody, IL-27A antibody, IL27A antibody, IL27p28 antibody, IL30 antibody, p28 antibody, RGD1561420 antibody, IL27 antibody, IL-27p28 antibody, Il30 antibody, interleukin 27 antibody, IL27 antibody, Il27 antibody

Background: IL-27 is a heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40-related protein) and p28 (a newly discovered IL-12 P35-related protein). It is a member of the IL-6/IL-12 cytokine family and mainly produced by antigen-presenting cells, including macrophages and dendritic cells. IL-27 acts on T cells and NK cells. It has been reported that IL-27 drives rapid clonal expansion of naive CD4+ T cells, and promotes Th1 polarization and IFN-γ production in synergy with IL-12. The IL-27-induced Th1 differentiation was mediated by rapid and marked upregulation of ICAM-1/LFA-1 interaction in a STAT1-dependent manner. IL-27 exhibits anti-inflammatory function by enhancing Th1 cell differentiation, a potent antitumor activity, through CD8+ T cell and NK cell activation. It also plays a potential therapeutic role in autoimmune disease by inhibiting Th-17 development. IL-27 mediates its biological effects through its receptor, WSX-1/T cell cytokine receptor (TCCR), which is homologous to the IL-12Rβ2 subunit. Protein gp130 serves as a functional signal-transducing molecule for IL-27.