MUC1 antibody

Catalog No. ABIN2689099

Product Details anti-MUC1 Antibody

Target: MUC1 (Mucin 1 (MUC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: Reacts with the core peptide of the MUC1 protein, a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200 kDa) and an amino acid composition rich in serine, threonin, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats which function as multiple epitopes for the monoclonal antibody. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This antibody has been shown to react with both normal and malignant epithelia of various tissues including breast and colon. Profile of U266 cells analyzed by flow cytometry

BD Pharmingen™ Purified Mouse Anti-Human CD227 - Purified - Clone HMPV - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: HMPV

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-MUC1 antibody (ABIN2689099)

Treon, Mollick, Urashima, Teoh, Chauhan, Ogata, Raje, Hilgers, Nadler, Belch, Pilarski, Anderson: "Muc-1 core protein is expressed on multiple myeloma cells and is induced by dexamethasone." in: Blood, Vol. 93, Issue 4, pp. 1287-98, (1999) (PubMed).

Nakamura, Hinoda, Nakagawa, Makiguchi, Itoh, Endo, Imai: "Detection of circulating anti-MUC1 mucin core protein antibodies in patients with colorectal cancer." in: Journal of gastroenterology, Vol. 33, Issue 3, pp. 354-61, (1998) (PubMed).

Reddish, MacLean, Koganty, Kan-Mitchell, Jones, Mitchell, Longenecker: "Anti-MUC1 class I restricted CTLs in metastatic breast cancer patients immunized with a synthetic MUC1 peptide." in: International journal of cancer. Journal international du cancer, Vol. 76, Issue 6, pp. 817-23, (1998) (PubMed).

Agrawal, Schatz: "RAG1 and RAG2 form a stable postcleavage synaptic complex with DNA containing signal ends in V(D)J recombination." in: Cell, Vol. 89, Issue 1, pp. 43-53, (1997) (PubMed).

Devine, Birrell, Whitehead, Harada, Xing, McKenzie: "Expression of MUC1 and MUC2 mucins by human tumor cell lines." in: Tumour biology, Vol. 13, Issue 5-6, pp. 268-77, (1993) (PubMed).

Xing, Prenzoska, Layton, Devine, McKenzie: "Second-generation monoclonal antibodies to intestinal MUC2 peptide reactive with colon cancer." in: Journal of the National Cancer Institute, Vol. 84, Issue 9, pp. 699-703, (1992) (PubMed).

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: CD227 (MUC1 Products)

Synonyms: CA 15-3 antibody, CD227 antibody, EMA antibody, H23AG antibody, KL-6 antibody, MAM6 antibody, MCKD1 antibody, MUC-1 antibody, MUC-1/SEC antibody, MUC-1/X antibody, MUC1/ZD antibody, PEM antibody, PEMT antibody, PUM antibody, Muc-1 antibody, mucin 1, cell surface associated antibody, mucin 1, transmembrane antibody, MUC1 antibody, Muc1 antibody

Background: Synonyms: MUC1

Pathways: Negative Regulation of intrinsic apoptotic Signaling