CD25 antibody

Catalog No. ABIN2689120

Product Details anti-CD25 Antibody

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

Brand: BD Pharmingen™

Characteristics: The Kei-α1 antibody reacts with the rabbit homolog of CD25, the interleukin-2 receptor alpha chain (IL-2Rα). mAb Kei-α1 recognizes specifically IL-2Rα and neither the IL-2R ß chain (CD122) nor the 25- kDa protein component of the rabbit high-affinity IL-2R complex. Based on flow cytometric analysis, fewer than 1 % of resting peripheral blood T and B cells have been found to stain with Kei-α1, while a majority of PHA blasts show intense staining with Kei-α1. The HTLV-1-transformed rabbit cell lines, F648b and YR-1 both also show intense staining with Kei-α1. mAb Kei-α1 has been reported to block both high-and low-affinity IL-2 binding and also cell proliferation of rabbit PHA blasts induced by picomolar concentrations of IL-2.

BD Pharmingen™ Purified Mouse Anti-Rabbit CD25 - Purified - Clone Kei-α1 - Isotype Mouse IgG2b, κ - Reactivity Rab - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Membrane preparation of the HTLV-1-transformed rabbit T-cell line F648b

Clone: Kei

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD25 antibody (ABIN2689120)

Kotani, Yamamura, Tsudo, Tamatani, Kitamura, Miyasaka: "Generation of monoclonal antibodies to the rabbit interleukin-2 receptor alpha chain (CD25) and its distribution in HTLV-1-transformed rabbit T cells." in: Japanese journal of cancer research : Gann, Vol. 84, Issue 7, pp. 770-5, (1993) (PubMed).

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25 (IL2RA Products)

Synonyms: CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody

Background: Synonyms: IL-2R alpha

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation