P-Selectin antibody

Catalog No. ABIN2689366

Product Details anti-P-Selectin Antibody

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This P-Selectin antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Brand: BD Pharmingen™

Characteristics: The RB40.34 antibody reacts with mouse P-selectin (CD62P), a 140 kDa protein which is expressed on activated platelets, activated endothelial cells, and megakaryocytes. P-selectin mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells, mediates leukocyte rolling, and is involved in the migration of leukocytes into inflamed tissues. CD24 and CD162 (PSGL-1) are ligands of CD62P. mAb RB40.34 can block mouse P-selectin binding to its ligands in vitro and in vivo. Immunohistochemical staining of megakaryocytes and platelets. The frozen section of normal mouse spleen was reacted with RB40.34 mAb. Megakaryocytes and platelets are identified by the intense brown staining.

BD Pharmingen™ Purified Rat Anti-Mouse CD62P - Purified - Clone RB40.34 - Isotype Rat IgG1, λ - Reactivity Ms - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: P-selectin-IgG1 Fusion

Clone: RB40-34

Isotype: IgG1 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 31.25 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-P-Selectin antibody (ABIN2689366)

Yang, Galipeau, Kozak, Furie, Furie: "Mouse P-selectin glycoprotein ligand-1: molecular cloning, chromosomal localization, and expression of a functional P-selectin receptor." in: Blood, Vol. 87, Issue 10, pp. 4176-86, (1996) (PubMed).

Aigner, Ruppert, Hubbe, Sammar, Sthoeger, Butcher, Vestweber, Altevogt: "Heat stable antigen (mouse CD24) supports myeloid cell binding to endothelial and platelet P-selectin." in: International immunology, Vol. 7, Issue 10, pp. 1557-65, (1996) (PubMed).

Ley, Bullard, Arbonés, Bosse, Vestweber, Tedder, Beaudet: "Sequential contribution of L- and P-selectin to leukocyte rolling in vivo." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 669-75, (1995) (PubMed).

Target Details for P-Selectin

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Alternative Name: CD62P (SELP Products)

Synonyms: PSELECT antibody, CD62 antibody, CD62P antibody, GMP140 antibody, GRMP antibody, LECAM3 antibody, PADGEM antibody, PSEL antibody, GMP-140 antibody, Grmp antibody, selectin P antibody, selectin, platelet antibody, Selp antibody, SELP antibody

Background: Synonyms: P-selectin