CD69 antibody (Biotin)

Catalog No. ABIN2689380

Product Details anti-CD69 Antibody (Biotin)

Target: CD69

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CD69 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The H1.2F3 antibody reacts with CD69 (Very Early Activation antigen), an 85 kDa disulfide-linked homodimer of differentially glycosylated subunits. CD69 is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. Its expression is rapidly induced upon activation of lymphocytes (T, B, NK, and NK-T cells), neutrophils, and macrophages. CD69 is expressed also on thymocytes that are undergoing positive selection, its role in that process is unclear. H1.2F3 mAb augments PMA-induced T-cell stimulation and IFN-γ-induced macrophage stimulation. IL-2-activated NK cells express CD69, and H1.2F3 mAb induces redirected lysis of FcR-bearing target cells by NK cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Hamster Anti-Mouse CD69 - Biotin - Clone H1.2F3 - Isotype Armenian Hamster IgG1, λ3 - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Dendritic Epidermal T Cell Line Y245

Clone: H1-2F3

Isotype: IgG1 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD69 antibody (Biotin) (ABIN2689380)

Lauzurica, Sancho, Torres, Albella, Marazuela, Merino, Bueren, Martínez-A, Sánchez-Madrid: "Phenotypic and functional characteristics of hematopoietic cell lineages in CD69-deficient mice." in: Blood, Vol. 95, Issue 7, pp. 2312-20, (2000) (PubMed).

Keefe, Dave, Allman, Wiest, Kappes: "Regulation of lineage commitment distinct from positive selection." in: Science (New York, N.Y.), Vol. 286, Issue 5442, pp. 1149-53, (1999) (PubMed).

Gabor, Godfrey, Scollay: "Recent thymic emigrants are distinct from most medullary thymocytes." in: European journal of immunology, Vol. 27, Issue 8, pp. 2010-5, (1997) (PubMed).

Marzio, Jirillo, Ransijn, Mauël, Corradin: "Expression and function of the early activation antigen CD69 in murine macrophages." in: Journal of leukocyte biology, Vol. 62, Issue 3, pp. 349-55, (1997) (PubMed).

Merkenschlager, Graf, Lovatt, Bommhardt, Zamoyska, Fisher: "How many thymocytes audition for selection?" in: The Journal of experimental medicine, Vol. 186, Issue 7, pp. 1149-58, (1997) (PubMed).

Punt, Suzuki, Granger, Sharrow, Singer: "Lineage commitment in the thymus: only the most differentiated (TCRhibcl-2hi) subset of CD4+CD8+ thymocytes has selectively terminated CD4 or CD8 synthesis." in: The Journal of experimental medicine, Vol. 184, Issue 6, pp. 2091-9, (1997) (PubMed).

Wilkinson, Anderson, Owen, Jenkinson: "Positive selection of thymocytes involves sustained interactions with the thymic microenvironment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 11, pp. 5234-40, (1995) (PubMed).

Ziegler, Ramsdell, Alderson: "The activation antigen CD69." in: Stem cells (Dayton, Ohio), Vol. 12, Issue 5, pp. 456-65, (1995) (PubMed).

Brändle, Müller, Müller, Hengartner, Pircher: "Regulation of RAG-1 and CD69 expression in the thymus during positive and negative selection." in: European journal of immunology, Vol. 24, Issue 1, pp. 145-51, (1994) (PubMed).

Ziegler, Levin, Johnson, Copeland, Gilbert, Jenkins, Baker, Sutherland, Feldhaus, Ramsdell: "The mouse CD69 gene. Structure, expression, and mapping to the NK gene complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1228-36, (1994) (PubMed).

Sobel, Yokoyama, Shevach, Eisenberg, Cohen: "Aberrant expression of the very early activation antigen on MRL/Mp-lpr/lpr lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 2, pp. 673-82, (1993) (PubMed).

Bendelac, Matzinger, Seder, Paul, Schwartz: "Activation events during thymic selection." in: The Journal of experimental medicine, Vol. 175, Issue 3, pp. 731-42, (1992) (PubMed).

Karlhofer, Yokoyama: "Stimulation of murine natural killer (NK) cells by a monoclonal antibody specific for the NK1.1 antigen. IL-2-activated NK cells possess additional specific stimulation pathways." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 10, pp. 3662-73, (1991) (PubMed).

Yokoyama, Maxfield, Shevach: "Very early (VEA) and very late (VLA) activation antigens have distinct functions in T lymphocyte activation." in: Immunological reviews, Vol. 109, pp. 153-76, (1989) (PubMed).

Yokoyama, Koning, Kehn, Pereira, Stingl, Coligan, Shevach: "Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 2, pp. 369-76, (1988) (PubMed).

Target Details for CD69

Target: CD69

Alternative Name: CD69 (CD69 Products)

Synonyms: 5830438K24Rik antibody, AI452015 antibody, AIM antibody, VEA antibody, BL-AC/P26 antibody, CLEC2C antibody, EA1 antibody, GP32/28 antibody, MLR-3 antibody, Cd69 molecule antibody, CD69 antigen antibody, CD69 molecule antibody, Cd69 antibody, CD69 antibody

Background: Synonyms: Very Early Activation antigen