The G265-8 antibody reacts with both the 72 and 77 amino acid forms of human interleukin-8 (IL-8). The immunogen used to produce the G265-8 hybridoma was E. coli-expressed recombinant human IL-8. Human IL-8 ELISA Standard Curve. Several distinct N-termini exist in the natural protein. The open square is a 72 amino acid recombinant protein and the filled circle is a 77 amino acid recombinant protein standard.
IL8
Reactivity: Human
WB, ELISA, IHC (p), IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Storage
4 °C
Storage Comment
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Matsushima, Oppenheim: "Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF." in: Cytokine, Vol. 1, Issue 1, pp. 2-13, (1991) (PubMed).