Recognizes Ki-67, a nuclear cell proliferation-associated antigen expressed in all active stages of the cell cycle. Ki-67 is revealed as a double band (345 and 395 kD) in western blot analysis of proliferating cells. B56 was developed using an immunogen composed of the immunodominant epitope of the Ki-67 protein. Antibodies B56 and MIB 1 react with this immunogen. Flow cytometric analysis reveals that the binding of B56-PE can be blocked by MIB 1 purified antibody. Immunohistochemistry analysis demonstrates a staining pattern similar to MIB 1 on both frozen and paraffin-embedded tissue sections. Immunohistochemistry of Ki-67 positive cells.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4°C.
Shi, Cui, Alam, Gunning, Nestor, Giovannucci, Zhang, Ding: "Nestin expression defines both glial and neuronal progenitors in postnatal sympathetic ganglia." in: The Journal of comparative neurology, Vol. 508, Issue 6, pp. 867-78, (2008) (PubMed).
Pecchi, Dallaporta, Charrier, Pio, Jean, Moyse, Troadec: "Glial fibrillary acidic protein (GFAP)-positive radial-like cells are present in the vicinity of proliferative progenitors in the nucleus tractus solitarius of adult rat." in: The Journal of comparative neurology, Vol. 501, Issue 3, pp. 353-68, (2007) (PubMed).
Target
Ki-67 (MKI67)
(Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))