alpha Adducin antibody
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- Target See all alpha Adducin (ADD1) Antibodies
- alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This alpha Adducin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of ADD1 protein. (regioin surrounding Lys439)
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Purity
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product ADD1 Primary Antibody
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- Application Notes
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ELISA: 1: 20000approx. 1: 40000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- DO NOT FREEZE!
- Storage
- 4 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C.
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- Target
- alpha Adducin (ADD1) (Adducin 1 (Alpha) (ADD1))
- Alternative Name
- alpha-Adducin (ADD1) (ADD1 Products)
- Synonyms
- ADDA antibody, AI256389 antibody, alpha-adducin antibody, bZ1H20.1 antibody, cb787 antibody, zgc:158352 antibody, add2 antibody, adda antibody, add1-a antibody, ADD1 antibody, adducin 1 antibody, adducin 1 (alpha) antibody, adducin 1 S homeolog antibody, ADD1 antibody, Add1 antibody, add1 antibody, add1.S antibody
- Background
- Adducins are a family of cytoskeleton proteins encoded by three genes (α, β, γ). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and Adducins are central in the cortical actin network organization. Adducin α is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin α and Adducin γ is ubiquitous in contrast to the restricted expression of Adducin β. Adducin β is expressed at high levels in brain and hematopoietic tissues, such as bone marrow, in humans, and in spleen in mice.Synonyms: ADDA, Erythrocyte adducin subunit alpha
- Molecular Weight
- approx. 81 kDa
- Gene ID
- 118
- NCBI Accession
- NP_001110
- UniProt
- P35611
- Pathways
- Negative Regulation of Hormone Secretion, Regulation of Actin Filament Polymerization, Regulation of Lipid Metabolism by PPARalpha, ER-Nucleus Signaling
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