CFTR antibody

Catalog No. ABIN272010

Product Details anti-CFTR Antibody

Target: CFTR (Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CFTR antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of CFTR protein. (region surrounding Glu733)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 1000approx. 1: 5000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CFTR

Target: CFTR (Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR))

Alternative Name: ABCC7 / CFTR (CFTR Products)

Synonyms: ABC35 antibody, ABCC7 antibody, CF antibody, CFTR/MRP antibody, MRP7 antibody, TNR-CFTR antibody, dJ760C5.1 antibody, CFTR antibody, AW495489 antibody, Abcc7 antibody, RGD1561193 antibody, abc35 antibody, abcc7 antibody, cftr/mrp antibody, mrp7 antibody, tnr-cftr antibody, xcftr antibody, si:dkey-270i2.2 antibody, cystic fibrosis transmembrane conductance regulator antibody, cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) antibody, CFTR antibody, cftr-A antibody, Cftr antibody, cftr antibody

Background: CFTR, for cystic fibrosis transmembrane conductance regulator, is a cyclic adenosine monophosphate (cAMP)-regulated chloride channel protein. CFTR belongs to the MDR subfamily within the ATP-binding transport protein family. It has two transmembrane domains (TMDs), two nucleotide binding domains (NBDs) and one regulatory domain. Mutations of CFTR are associated with cystic fibrosis (CF), a disease characterized by chronic bronchopulmonary disease, elevated sweat electrolytes and insufficient pancreatic function. CFTR mutations can also result in congenital bilateral absence of vas deferens (CBAVD), a form of male sterility that a majority of male CF patients exhibit. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86 % and 100 % reductions in S. typhi intestinal submucosal uptake.Synonyms: ATP-binding cassette transporter sub-family C member 7, Channel conductance-controlling ATPase, Cystic fibrosis transmembrane conductance regulator, cAMP-dependent chloride channel

Molecular Weight: approx. 168 kDa

Gene ID: 1080

NCBI Accession: NP_000483

UniProt: P13569