CDK8 antibody

Catalog No. ABIN272196

The Rabbit Polyclonal anti-CDK8 antibody (ABIN272196) specifically detects CDK8 in WB and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for CDK8 antibody (ABIN272196)

Target: CDK8 (Cyclin-Dependent Kinase 8 (CDK8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDK8 Antibody

Specificity: This antibody detects endogenous levels of Cdk8 protein. (region surrounding His34)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CDK8

Target: CDK8 (Cyclin-Dependent Kinase 8 (CDK8))

Alternative Name: CDK8

Background: Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression.Another family of proteins, Cdk inhibitors, also plays a role in regulating cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-3, PITALRE and PITSLRE. Large complexes containing Cdk8, cyclin C and the large subunit of RNA polymerase II have been identified. Cdk8 is thought to regulate RNA polymerase II function in conjunction with cyclin C. Cdk8 has been demonstrated to function as a transcriptional activator when fused to the DNA binding domain of GAL4.Synonyms: Cell division protein kinase 8, Cyclin-dependent kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Protein kinase K35

Molecular Weight: approx. 55 kDa

Gene ID: 1024

NCBI Accession: NP_001251

UniProt: P49336

Pathways: Cell Division Cycle, Regulation of Lipid Metabolism by PPARalpha