TAF2 antibody (TAF2 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 150kDa) (Middle Region)

Details for Product anti-TAF2 Antibody No. ABIN2777909, Supplier: Log in to see
Antigen
  • CG6711
  • Dmel\\CG6711
  • TAF
  • TAF150
  • TAF2
  • TAFII150
  • TAF[II]150
  • TAF[[II]]
  • TAF[[II]]150
  • TFIID
  • Taf150
  • TafII150
  • Taf[[II]]150
  • dTAF II 150
  • dTAF150
  • dTAFII150
  • dTAF[[II]]150
  • TAFII-150
  • fc30f02
  • wu:fc30f02
  • 150kDa
  • 4732460C16Rik
  • AI425886
  • CIF150
  • TAF2B
  • TAFIIB
  • TBP-associated factor 2
  • TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor
  • TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa
  • Taf2
  • taf2
  • TAF2
Epitope
Middle Region
14
10
9
3
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
30
16
6
6
3
3
2
2
2
2
1
1
1
1
1
Host
Rabbit
37
5
Clonality
Polyclonal
Conjugate
This TAF2 antibody is un-conjugated
2
2
2
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
31
22
20
17
4
4
1
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the middle region of human TAF2
Blocking Peptide Blocking peptide for this product available: ABIN984730
Sequence RKRNVLELEI KQDYTSPGTQ KYVGPLKVTV QELDGSFNHT LQIEENSLKH
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%
Characteristics This is a rabbit polyclonal antibody against TAF2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name TAF2 (TAF2 Antibody Abstract)
Background Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF2 is one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators.Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CIF150, TAF2B, TAFII150
Protein Interaction Partner: SHFM1, SOX2, SIRT1, RNPS1, MED26, HIST3H3, TAF9, TAF6, TAF1, ISG15, ESR1, TAF10, ELAVL1, UBC, TAF8, TERF2, TERF1, TBP, KDM5B, TAF13, TAF12, TAF11, TAF5, TAF4, TAF7,
Protein Size: 1199
Molecular Weight 137 kDa
Gene ID 6873
NCBI Accession NM_003184, NP_003175
UniProt Q6P1X5
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1199 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Chromatin Immunoprecipitation (ChIP) image for anti-TAF2 antibody (TAF2 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 150kDa) (Middle Region) (ABIN2777909) Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three t...
Western Blotting (WB) image for anti-TAF2 antibody (TAF2 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 150kDa) (Middle Region) (ABIN2777909) WB Suggested Anti-TAF2 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:12500 Positive ...
Background publications Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

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